bsdB [2019-06-19 11:10:32]

phenolic acid decarboxylase

locus: BSU03630

pI: 10.06

mw: 22.39 kDa

protein length: 204 aa

gene length: 615 bp

function: resistance to salicylic acid

product: phenolic acid decarboxylase

essential: no

synonyms: yclB

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.10|Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)]

Gene

Coordinates: 412,540 413,154

The protein

Catalyzed reaction/ biological activity

• conversion of vanillic acid to guaiacol [Pubmed|26658822]

Protein family

• polyprenyl P-hydroxybenzoate / phenylacrylic acid decarboxylases family (according to Swiss-Prot)

Structure

• [PDB|1SBZ] (from E. coli, 57% identity) [pubmed|15459342]

Expression and Regulation

Operons

• genes: [gene|8A83E44AFE9A1A635C65E6B526690AF7DA201BE1|bsdB]-[gene|FD23F4C2AFCBFE35A79116C5A707FACD454E4180|bsdC]-[gene|868DC9DE1C9C26C780F4CEAA4185640B5B6B781B|bsdD]
  description: [Pubmed|17295427]

  regulatory mechanism

  • [protein|28D03575FA24525C162E6C161631034568E89622|BsdA]: activation, [Pubmed|17295427], in [regulon|28D03575FA24525C162E6C161631034568E89622|BsdA regulon]

  regulation

  • induced by salicylic acid ([protein|search|BsdA]) [Pubmed|17295427]

Biological materials

Mutant

• MGNA-C061 (yclB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2059 NBRP B. subtilis, Japan]
• BKE03630 ([gene|8A83E44AFE9A1A635C65E6B526690AF7DA201BE1|bsdB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCAATCATACTCCTGA, downstream forward: _UP4_AAACAAAAAGGAGGAGCTTG
• BKK03630 ([gene|8A83E44AFE9A1A635C65E6B526690AF7DA201BE1|bsdB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCAATCATACTCCTGA, downstream forward: _UP4_AAACAAAAAGGAGGAGCTTG

References

• 15979273,17295427,26658822,15459342